Fig. 1

Tumors with promoter DNA methylation and reduced expression of Polg have reduced mitochondrial DNA content. a qMSP of bisulfite-treated DNA from indicated tissue 4 or 8 weeks post-infection using methylation-specific primers in promoter CpG islands of indicated genes. Mean ± SEM. N = 4–5 for epithelium, N = 5–9 for tumors. *P < 0.05 compared with mock epithelium. ^#P < 0.05 compared to ETBF Min epithelium and mock Min and ETBF Msh2^l/lVCMin tumors. b Polg gene expression by qPCR relative to mock epithelium. Mean ± SEM. N = 4–8 for epithelium, N = 6–12 for tumors, asterisk and number sign as in a. DNA from tissue (c) 4 weeks or (d) 8 weeks post-infection was used in qPCR assays with primer sets specific for two different regions of mitochondrial DNA or the genomic ApoB. Mean ± SEM. N = 4–5 for epithelium, N = 5–9 for tumors, asterisk and number sign as in a