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Table 3 Antibodies for Western blot

From: Beta-hydroxybutyrate (3-OHB) can influence the energetic phenotype of breast cancer cells, but does not impact their proliferation and the response to chemotherapy or radiation

Antibody

Gen/name

Company

Clone

Species

Dilution

MW of antigen

Primary

Beta-actin

Abcam

mAbcam 8226

Mouse

1:10000

42 kDa

Monocarboxylic acid transporter 2 (MCT-2)

Abcam

Polyclonal

Rabbit

1:2000

52 kDa

Glucose transporter 1 (GLUT1)

USBiological

Polyclonal

Rabbit

1:1000

55 kDa

Secondary

HRP-labeled goat-anti-mouse

KPL

Polyclonal

Goat

1:10000

 

HRP-labeled goat-anti-rabbit

Abcam

Polyclonal

Goat

1:20000

 
  1. MW molecular weight