Fig. 5

PKM2-deficient sarcoma tumors have increased proliferation and an altered metabolic profile. a Representative images of dual-color IHC for PKM1 or PKM2 (brown) and pHH3 (purple) on KP M2^+/+ and KP M2^−/− sarcoma tissue. Scale bars, 20 μm. b Representative images of IF for tdTomato, PKM1, and EdU on KP M2^−/− sarcoma tissue. c Quantification of pHH3+ cells in KP M2^+/+ and KP M2^−/− sarcomas. Representative images of the IHC for pHH3 are shown. Scale bars, 20 μm, n = 6 tumors per genotype. p value using unpaired t test is shown, *p < 0.05. d Heatmap showing measured metabolites that were significantly different between KP M2^+/+ and KP M2^−/− sarcoma tissue. A full list of the measured metabolites is included in Additional file 4: Table S1