Fig. 4

PKM2-deficient sarcoma tumor cells express PKM1. a Western blot analysis for PKM1 and PKM2 on lysates from KP M2^+/+ or KP M2^−/− sarcoma tissue. Hsp90 was used as a loading control. b Expression of Pkm1, Pkm2, and total Pkm mRNA in KP M2^+/+, KP M2^−/−, or wild-type skeletal muscle tissue was assessed by qRT-PCR. Mean values ± SEM and p values using unpaired t test are shown, *p < 0.05; **p < 0.01; ****p < 0.0001. c Pyruvate kinase enzymatic activity of KP M2^+/+, KP M2^−/−, or wild-type skeletal muscle tissue, p value using unpaired t test is shown, *p < 0.05. d Representative images for PKM1 and PKM2 IHC in KP M2^+/+ and KP M2^−/− sarcoma tissue. Asterisks (*) indicate skeletal muscle fibers. Scale bars, 20 μm. e Representative images of IF for tdTomato and PKM2 on KP M2^−/− sarcoma tissue