Fig. 4

IDH1 mutant cells show diminished vulnerability to radiation. a–c Assessment of DNA damage and repair by comet analysis following irradiation (10 Gy). a Representative images of cells following comet assay. White arrows indicate cells with comets. b Graph showing the percent of cells with comets. Comet number was calculated for each line individually and then averaged by group. c Tail moment length for each comet was calculated as the length from the center of the comet head to the center of the tail. Tail moment length was calculated for each line individually and then averaged by group. Significance levels were calculated by ANOVA followed by post hoc t tests. Asterisks denote p < 0.05, error bars: ± SEM. d Quantification of the effects of increasing doses of radiation (0, 2, 6, and 10 Gy) on culture growth as described in the “Methods” section. Graphs represent cell counts normalized to non-irradiated controls