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Fig. 3 | Cancer & Metabolism

Fig. 3

From: Metabolic characterization of isocitrate dehydrogenase (IDH) mutant and IDH wildtype gliomaspheres uncovers cell type-specific vulnerabilities

Fig. 3

IDH1 mutant cells utilize de novo and salvage pathways for nucleotide biosynthesis. a, b Quantification of de novo nucleotide synthesis and salvage pathway utilization by LC-MS with labeled glucose and nucleotides. a The relative contribution of each pathway plotted in bar graph format. b The same data plotted two-dimensionally. Error bars represent ± STDEV. c Cell cycle analysis by flow cytometry (DAPI) following 4-day treatment with 1 mM deoxythymidine (dT) or control (left: distribution of cells in G1, S phase, and G2; right: representative histograms of cell cycle distribution for IDH1 mutant and WT cultures following dT treatment)

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Cancer & Metabolism

ISSN: 2049-3002