Fig. 1

KEGG Gene Set Enrichment Analysis. Plot of expression data from TCGA (167 KEGG modules) and our gliomasphere dataset (186 KEGG modules). Each KEGG module was assigned a normalized enrichment score (NES) in either the IDH1 mutant or IDH1WT group. Each blue dot represents a different module. The enrichment score for a particular module in the TCGA data is plotted along the X-axis and that associated with the gliomasphere dataset for the same module is plotted on the Y-axis. Thus, a module that is highly enriched in IDH1 mutants compared to wildtype in both datasets would be in the upper right corner and modules highly enriched in IDH wildtype in both datasets would be in the lower left hand corner. Differentially enriched modules (NES > 1.2), listed on the right, were identified as potential candidates for further investigation