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Fig. 2 | Cancer & Metabolism

Fig. 2

From: Cancer cell metabolic plasticity allows resistance to NAMPT inhibition but invariably induces dependence on LDHA

Fig. 2

Characterization of FK866-resistant cells. a, b Administration of FK866 decreases intracellular ATP and NAD(H) levels in CEM PA cells. CEM PA and CEM RES cells were treated with 5 and 100 nM FK866 for 48 h. Relative ATP and NAD(H) levels were normalized to number of viable cells. c FK866 inhibits global protein translation. CEM PA and CEM RES cells were treated with 100 nM FK866 for 48 h. Protein synthesis was monitored by Click-it chemistry based on the incorporation of an amino acid analog (AHA). The histogram quantifies FK866-induced protein synthesis arrest in the viable cell population. d Time course of the NAMPT enzymatic activity assayed in lysates from CEM PA and CEM RES cells. Assays were performed in the absence and in the presence of 1 μM FK866 in the reaction mixtures. e NAMPT enzymatic activity was analyzed in lysates from CEM RES cells in the absence and in the presence of the indicated FK866 concentrations. All data were conducted in three independent experiments with technical triplicates (*p < 0.05, **p < 0.01, ***p < 0.001 compared to Mock)

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