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Table 1 Kinetic parameters for MTHFD2 5,10-CH2-THF dehydrogenase activity. 5,10-CH2-THF kinetic parameters were determined using saturating concentrations of NAD+ (1.0 mM) or NADP+ (6.0 mM). When NAD+ was used, potassium phosphate (25 mM) and MgCl2 (5 mM) were also included

From: Human mitochondrial MTHFD2 is a dual redox cofactor-specific methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase

  CH2-H4PteGlu1 CH2-H4PteGlu5
  KM (μM) kcat (s−1) kcat/KM (s−1 μM−1) KM (μM) kcat (s−1) kcat/KM (s−1 μM−1)
NAD+-dependent 133 ± 20 12.4 ± 0.71 0.093 359 ± 32 15.4 ± 0.55 0.043
NADP+-dependent 123 ± 24 1.5 ± 0.11 0.012 302 ± 35 6.4 ± 0.29 0.021