Table 1 Kinetic parameters for MTHFD2 5,10-CH₂-THF dehydrogenase activity. 5,10-CH₂-THF kinetic parameters were determined using saturating concentrations of NAD⁺ (1.0 mM) or NADP⁺ (6.0 mM). When NAD⁺ was used, potassium phosphate (25 mM) and MgCl₂ (5 mM) were also included

From: Human mitochondrial MTHFD2 is a dual redox cofactor-specific methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase