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Fig. 3 | Cancer & Metabolism

Fig. 3

From: Bortezomib resistance in multiple myeloma is associated with increased serine synthesis

Fig. 3

Enhanced pentose phosphate pathway activity increases the anti-oxidant capacity of bortezomib-resistant cells. a Total GSH levels of RPMI-8226 wild type (WT) and bortezomib resistant (BTZ/100) cells. Data are means ± SD (n = 3) b Cell viability of RPMI-8226 WT and BTZ/100 cells after a 16-h treatment with increasing concentrations of H2O2. Results represent mean cell viability ± SD compared to non-treated controls (n = 3). c Cell viability of RPMI-8226 BTZ/100 cells after treatment for 24 h with 50 μM trans-androsterone (TA), followed by 24 h with increasing concentrations of H2O2. Results represent mean cell viability ± SD compared to non-treated control (n = 3). d GSH/GSSG ratio of RPMI-8226 WT and BTZ/100 cells after 5 min treatment with 5 mM DHA. Data are means ± SD (n = 3). e Analysis of intracellular levels of DHA (left panel) and AA (right panel) in RPMI-8226 WT and BTZ/100 cells. Cells were treated with 5 mM DHA or vehicle for 5 min and subjected to LC-MS analyses. Data are means ± SD (n = 3). Two-way ANOVA tests were performed (ns = not significant, * = p < 0.05). AA = ascorbic acid, DHA = dehydroascorbic acid, GSH = glutathione, TA = trans-androsterone

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