Fig. 5

RTK-dependent TNBC sensitization to metabolic pathway perturbation. a Metabolic rate response to RTK inhibitors. 40,000 cells per well were plated in Seahorse 96-well assay plates. Cells were treated for 24 h with the DMSO, erlotinib, or INC280, and then ECAR and OCR were measured as described in Methods. b Principle component analysis of drug responses. Mesenchymal-like subtype MDA-MB-231 and Hs578 cell lines show largest perturbations. c RNAi screen results for selected TKI treatments which induce sensitivities to knockdown of common metabolic pathway genes