Fig. 3

TNBC maximal metabolic profiles. a Schematic indicating derivation of maximal metabolic rates, glycolytic reserve, and spare respiratory capacity from the experiments in (a) and (b). b Cells were maintained in uniform media prior to measuring ECAR. ECAR was measured twice in the basal state, and then twice again following each metabolic inhibitor treatment (1 μM FCCP, 50 mM 2-deoxyglucose) at 7 minute intervals. ECAR values were normalized to a measurement of total cell numbers for each cell line in the ECAR assay. Data are ECAR averages from one representative experiment, with error bars representing SD. c Cells were maintained in uniform media prior to measuring OCR. OCR was measured twice in the basal state, and then twice again following each metabolic inhibitor treatment (1 μM FCCP, 1 μM rotenone + 1 μM antimycin) at 7 minute intervals. OCR values were normalized to a measurement of total cell numbers for each cell line in the OCR assay. Data are OCR averages from one representative experiment, with error bars representing SD. d Glycolytic reserve (derived from b) and spare respiratory capacity (derived from c) calculations. Data are expressed as actual rate unit increase and percent increase over basal rates. Rate increases were calculated by subtracting the basal rate values from the maximal rate values. Percent increases were calculated by dividing the rate increase values by the basal values