Fig. 2

TNBC basal metabolic profiles. a Cells were maintained in uniform media for 24 h prior to measuring ECAR. ECAR values were normalized to total cell numbers for each cell line in the ECAR assay. Data are ECAR averages from four experiments, each with five biological replicates. Error bars represent SEM. Asterisks indicate significance compared to HME1 cell values (p ≤ 0.05). b Cells were maintained in uniform media for 24 h prior to measuring OCR. OCR values were normalized to total cell numbers for each cell line in the OCR assay. Data are OCR averages from four experiments, each with three biological replicates. Error bars represent SEM. Asterisks indicate significance compared to HME1 cell values (p ≤ 0.05). c Relative ECAR and OCR data from a and b were plotted simultaneously to reveal overall relative basal metabolic profiles for each cell model. d The ECAR/OCR ratio of the data in d was log2 transformed to provide an index of each cell model’s comparative utilization of glycolytic and oxidative metabolism