Fig. 4
From: Mitochondrial mutations and metabolic adaptation in pancreatic cancer
Metabolic pathway flux analysis: a. Heirarchical clustering of significantly altered metabolic pathways (ANOVA, p < 0.05) in 13 PDCLs relative to normal HPDE cells (in K-SFM media), identified by PAPi analysis. Various culture media are represented by colours along the top of columns (grey = M199/F12, green = RPMI, purple = HPAC modified, red = IMDM). b Conversion of glutamine to lipid by 14C glutamine tracing in PDCLs cultured under normoxic and hypoxic conditions. c Metabolomics data are consistent with the activation of reductive carboxylation in pancreatic tumours, driving conversion of glutamine to lipid in the presence of low oxygen and ETC inhibition