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Fig. 4 | Cancer & Metabolism

Fig. 4

From: Adipocyte lipolysis links obesity to breast cancer growth: adipocyte-derived fatty acids drive breast cancer cell proliferation and migration

Fig. 4

MCF-7 cells have greater CPT1 protein levels and fatty acid oxidation rates compared to MDA-MB-231 cells, and these are increased high lipid environments. a CPT1A expression in a range of normal, luminal, and basal breast cancer cell lines. b [1-14C]-palmitate oxidation, c representative immunoblots of three independent experiments, and d densitometric quantitation of CPT1A protein levels in MCF-7 and MDA-MB-231 cells in the basal state and after 24 h culture in 0.3 mM oleate. e Oncoprint output showing frequency of CPT1A alteration in a TCGA cohort of ER+ breast cancer (n = 594) (red rectangle, amplification; light red rectangle, mRNA upregulation; black rectangle, truncated mutation; green rectangle, missense mutation; gray rectangle, unaltered). f Overall survival among ER+ breast carcinoma TCGA cases based on CPT1A alterations. g Representative immunoblots of CPT1A and protein subunits in the mitochondrial complexes (complex II-30 kDa, complex III-Core protein 2, complex IV-subunit 1, and complex V-alpha subunit) in MCF-7 and MDA-MB-231 cells co-cultured with or without lean or obese 3T3-L1 adipocytes (three independent experiments performed in duplicate). Data are presented as mean ± SEM. *P ≤ 0.05 by two-way ANOVA followed by Tukey’s multiple comparisons test

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