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Fig. 3 | Cancer & Metabolism

Fig. 3

From: Adipocyte lipolysis links obesity to breast cancer growth: adipocyte-derived fatty acids drive breast cancer cell proliferation and migration

Fig. 3

Altered glucose and glutamine metabolism in MDA-MB-231 and MCF-7 cells following co-culture with lean and obese adipocytes a MCF-7 cells and b MDA-MB-231 cells [U-14C]-glucose metabolism including total uptake (sum of media 14CO2, 14C activity in both the aqueous and organic phases of a Folch extraction), incorporation into intracellular lipids, 14CO2 generation (oxidation), and incorporation into DNA/RNA after co-cultured with or without 3T3-L1 adipocytes for 48 h (three independent experiments performed in duplicate, relative to cells in isolation (dotted line)). c MCF-7 cells and d MDA-MB-231 cells [1-14C]-l-glutamine metabolism after co-cultured with or without 3T3-L1 adipocytes for 48 h (three independent experiments performed in duplicate, relative to cells in isolation (dotted line)). e, f Absolute rates of 14C-labeled substrate incorporation into intracellular lipids (lipid synthesis) and total uptake (sum of media 14CO2, 14C activity in both the aqueous and organic phases of a Folsch extraction) of various substrates in MCF-7 (e) and MDA-MB-231 (f) cells and g percent contribution of substrates to lipid synthesis in both cell lines in the basal state (three independent experiments performed in duplicate). Data are presented as mean ± SEM. ad *P ≤ 0.05 compared to isolation; #P ≤ 0.05 compared to lean by one-way ANOVA followed by Tukey’s multiple comparisons test. ef *P ≤ 0.05 compared to oleate; #P ≤ 0.05 compared to glucose by one-way ANOVA followed by Tukey’s multiple comparisons test

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