Fig. 2

Adipocytes alter fatty acid partitioning in breast cancer cells. a MCF-7 cells and b MDA-MB-231 cells [1-¹⁴C]-oleate metabolism including total uptake (sum of media ¹⁴CO₂, ¹⁴C activity in both the aqueous and organic phases of a Folch extraction), incorporation into intracellular lipids (storage), and ¹⁴CO₂ generation (oxidation) after co-culture with or without 3T3-L1 adipocytes for 48 h (three independent experiments performed in triplicate). c MCF-7 cells and d MDA-MB-231 cells [1-¹⁴C]-oleate metabolism after co-culture with or without differentiated human primary mammary pre-adipocytes for 48 h (two independent experiments performed in duplicate). Data are presented as mean ± SEM, relative to cells in isolation (dotted line). *P ≤ 0.05 compared to isolation; #P ≤ 0.05 compared to lean by one-way ANOVA followed by Tukey’s multiple comparisons test