Fig. 6

XAV939 promotes colon cancer cell recovery from 3-bromopyruvate. a Experimental workflow for colorimetric-based survival curve assay. Individual cell lines were seeded on day 0 and treated after 24 h (day 1) with 200, 250, or 300 μM 3-bromopyruvate +/− Wnt signaling inhibitor XAV939 (10 μM) until day 5. On day 5 treatments were “washed out” and replaced with control medium. Cells were fixed on days 5–10 post seeding to observe recovery over time. SW480 (b), HCT116 (d) cells treated with the Wnt signaling inhibitor XAV939 (10 μM) survived and recovered compared to without treatment of 3-bromopyruvate. SW620 (c) cells recovered similarly +/− XAV939 treatment, and DLD1 cells (e) did not recover. Graphs shown represent the average of three independent replicates (+/− SEM) (*p value < 0.05; **p value < 0.01; ***p value < 0.001)