Fig. 2
Wnt signaling correlates with MCT-1 levels and activity in colon cancer cells. a Luciferase reporter activity in parental SW480, SW620, HCT116, and DLD-1 cells shows varying levels of Wnt signaling based on SuperTopflash activity. Graph represents the average of three trials (+/− SEM). (*p value < 0.05; **p value < 0.01; ***p value < 0.001). b qRT-PCR analysis was performed on RNA collected from parental SW480, SW620, HCT116, and DLD-1 cells. Graph represents the average of three trials with fold change over SW480 cells (+/− SD). c Whole cell lysates from each cell line were collected and probed with the antibodies shown. d Radiolabeled 14C pyruvate uptake assay on SW480 cells treated with Wnt signaling inhibitor XAV939 (72 h) or vehicle (DMSO). Graph represents average of n = 4 with the shaded area showing +/− SEM (*p value < 0.05; **p value < 0.01; ***p value < 0.001)