Cancer & Metabolism

Table 1 Metabolites detected in 10 μl cell culture medium measured by GC-MS

From: Fast exchange fluxes around the pyruvate node: a leaky cell model to explain the gain and loss of unlabelled and labelled metabolites in a tracer experiment

Metabolite	DMEM	DMEM and serum	HPDE cells	PDAC cells
Lactate	−	+	+++	+++
Pyruvate	+	+	+++	+++
Succinate	−	+	−	+
2-oxoglutarate	+	+	++	−
Malate	−	++	+	++
Citrate	−	+++	+	++
Glycerol	+	+++	+	++
Alanine	−	+	++	++
Aspartate	−	+	+	+
Glutamate	−	+	++	++
Glutamine	+	++	+	+
Glycine	+	+	+	++
Serine	+	+	+	+
Proline	−	+	+	++

Concentrations were scored qualitatively with “+”, “++” and “+++” to show relative quantity. Metabolites with peak area less than 5 % of the largest peak are marked with “−”

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