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Fig. 6 | Cancer & Metabolism

Fig. 6

From: Fast exchange fluxes around the pyruvate node: a leaky cell model to explain the gain and loss of unlabelled and labelled metabolites in a tracer experiment

Fig. 6

Extracellular metabolites and flux changes in PANC-1 cells treated with TNF-α. Bars show abundance of unlabelled (white) and fully labelled (grey) fractions. Trend lines show fitting of simulated data to experimental data generated by constrained non-linear least-square regression. a Metabolic model used to simulate data to fit experimental data. The model included glycolysis, glutaminolysis, PPP, TCA cycle, and the reversible exchange of lactate and pyruvate between intracellular and extracellular pools (see Additional file 1: S8). b Concentration profiles of extracellular pyruvate and lactate. c Concentration profile of glucose. d Estimated fluxes showing metabolic differences in PANC-1 cells with and without TNF-α as treatment. Flux intervals were generated by a Monte Carlo approach. netLac net lactate efflux, PK pyruvate kinase, ME1 cytoplasmic NADP-dependent malic enzyme, PT mitochondrial pyruvate carrier, _c control, TNF-α

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