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Fig. 3 | Cancer & Metabolism

Fig. 3

From: Fast exchange fluxes around the pyruvate node: a leaky cell model to explain the gain and loss of unlabelled and labelled metabolites in a tracer experiment

Fig. 3

Enrichment of extracellular metabolites of HPDE and PANC-1 cells on DMEM labelled with [U-13C6]-glucose. Bar graphs show fractional labelling of metabolites measured in triplicates. Alanine enrichment was used as proxy for mitochondrial pyruvate. Acetyl-CoA enrichment was calculated from malate and citrate enrichment. Glucose carbon atoms appear to overflow into lactate, with a small portion entering TCA cycle. Heat map showed a greater dilution of glucose carbons (i.e., loss of 13C enrichment) in PANC-1 cells compared to HPDE cells. Asterisk indicates p < 0.05

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Cancer & Metabolism

ISSN: 2049-3002