Fig. 5
From: Bridging the gap between non-targeted stable isotope labeling and metabolic flux analysis
Effects of siRNA-mediated silencing of NAT8L. Transfection of A549 cells with NAT8L targeting siRNA (siNAT8L) leads to a significant reduction of a NAA and b NAAG levels, and c an increase in β-CG as compared to transfection with a non-targeting siRNA (siCtrl) or untreated cells (Ctrl). NAAG levels after NAT8L silencing were below the detection limit (n.d.: not determinable). d Proliferation of A549 cells is significantly reduced upon transfection with NAT8L targeting siRNA (siNAT8L) as compared to transfection with a non-targeting siRNA (siCtrl). Cell numbers 72 h after siRNA transfection are normalized to average of siCtrl. e Proliferation of hepatocellular carcinoma cells (JHH-4), bronchial epithelial cells (BEAS-2B), and non-neoplastic hepatocytes (PH5CH8) is significantly reduced upon transfection with NAT8L targeting siRNA (siNAT8L) as compared to transfection with a non-targeting siRNA (siCtrl). Cell numbers 72 h after siRNA transfection are normalized to average of siCtrl. Data are presented as means ± standard deviation of n=3 replicates (a–c) or three independent experiments with three technical replicates (n = 9); asterisks indicate Welsh’s t test p values of below 0.05 (*) or 0.01 (**)