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Fig. 7 | Cancer & Metabolism

Fig. 7

From: Metabolic changes associated with methionine stress sensitivity in MDA-MB-468 breast cancer cells

Fig. 7

Methionine stress induces an increase in oxidative stress. a Two-photon fluorescence intensity images of individual MB468 and MB468res-R8 cells in Met+ or Met-Hcy+ media (48 h). Pixels within three groups in the phasor histogram have been isolated and colored accordingly in the FLIM map image. Higher free/bound NADH ratios are highlighted in green, indicating a more reduced state in the nucleus. Lower free/bound NADH ratios are highlighted in blue, indicating higher ratios of oxidative phosphorylation versus glycolysis in the mitochondria/cytoplasm. Long lifetime species (LLS) are highlighted in red, indicating the presence of oxidized lipids in the cell. b Phasor histograms of MB468 and MB468res-R8 cells. The black box selects the phasors with LLS signature. c A boxplot representing the percentage of pixels with the LLS (oxidized lipids) signature in MB468 (blue) and MB468res-R8 (yellow) in in Met-Hcy+ over a 48 h time course

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