Fig. 4
From: Metabolic changes associated with methionine stress sensitivity in MDA-MB-468 breast cancer cells
Metabolite changes linked to methionine and nucleoside metabolism. a MB468 and MB468res-R8 cells were cultured in Met+ or Met-Hcy+ media over the course of 24 h and analyzed by untargeted GCTOFMS and HILIC-UHPLC-qTOFMS-based metabolomics. Heatmaps represent fold changes of metabolites in the purine salvage as compared to the Met+, time-zero sample. Metabolites are color filled by fold change: yellow indicates a decrease in metabolite abundance and blue indicates an increase as compared to time-zero control. b Schematic of polyamine synthesis and methionine regeneration by way of the methionine salvage pathway. Metabolites with gray arrows are pointing to line graphs of the corresponding metabolite abundances in MB468 (blue line with squares) and MB468res-R8 (black line with circles) cells. Peak height reflecting relative metabolite abundance is marked on the y-axis and hours in homocysteine are marked on the x-axis. Error bars represent standard deviation. c Heatmaps represent fold changes of metabolites involved in purine/pyrimidine synthesis. Color fill is the same as a. d Schematic of purine synthesis and pyrimidines with corresponding metabolite abundances, same as b