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Fig. 3 | Cancer & Metabolism

Fig. 3

From: Metabolic changes associated with methionine stress sensitivity in MDA-MB-468 breast cancer cells

Fig. 3

Homocysteine media induces a metabolic response in MB468 and MB468res-R8 cells. MB468 and MB468res-R8 cells were cultured in Met+ or Met-Hcy+ media over the course of 24 h and analyzed by untargeted UHPLC- and GCMS-based methodologies (see “Methods” section). a Heatmaps representing metabolites measured in both MB468 and MB468res-R8 cells. Metabolites are color filled based on fold change to the Met+, time-zero sample. Yellow indicates a decrease in metabolite abundance and blue indicates an increase as compared to time-zero control. b, c Pathways enriched within 4 h of culturing in Met-Hcy+ media. Metabolites with significantly different abundance at 2 and 4 h as compared to time zero were determined using the Bayesian t test, Cyber-T [37, 38], with a Benjamini-Hochberg p ≤ 0.05. Enriched pathways were determined using the web server Metabolite Biological Role (MBRole) with a raw p value cutoff at p ≤ 0.1 [39]

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