Fig. 7
From: The role of HIF-1 in oncostatin M-dependent metabolic reprogramming of hepatic cells
OSM does not influence PDK1 levels in the two HCC cell lines. Cells were treated with OSM (50 ng/ml), hypoxia 1 % O2, a combinatorial treatment for the given time points. a, d qRT-PCR of PDK1 and PDP2 after the indicated period of time and treatment, mRNA from three biological replicates in HepG2 (a) and JHH-4 (d) cells. Fold change was calculated to the untreated control. b, e Western blot analysis of all three PDH phosphorylation sites (S232, S293, S300) and PDK1 in HepG2 (b) and JHH-4 (d) cells. α-Tubulin was used as a loading control and one representative α-tubulin blot is shown. c, f PDK1 protein levels in HepG2 (c) and JHH-4 (f) cells after the indicated period of time and treatment, shown as percentage of the untreated control. All error bars indicate the standard deviation calculated from three biological replicates. Statistical significance was determined in comparison to the untreated control. * P<=0.05, ** P<=0.01, and *** P<=0.001