Fig. 3
From: The role of HIF-1 in oncostatin M-dependent metabolic reprogramming of hepatic cells
PDK1 levels are increased upon OSM stimulation under normoxia in PH5CH8 non-neoplastic hepatocytes. PH5CH8 cells were treated with OSM (50 ng/ml), hypoxia (1 % O2), or a combinatorial treatment for the indicated periods of time. a Quantitative RT-PCR of PDK1 and PDP2 mRNA. The fold change was calculated relative to the untreated control. b Western blot analysis for all three PDH phosphorylation sites (S232, S293, S300) and PDK1. α-Tubulin was used as a loading control, and one representative α-tubulin blot is shown. c Quantification of PDK1 protein levels, shown as percentage of the untreated control. All error bars indicate the standard deviation from three biological replicates. Statistical significance was determined in comparison to the untreated control. * P<=0.05, ** P<=0.01, and *** P<=0.001