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Fig. 5 | Cancer & Metabolism

Fig. 5

From: Targeting mitochondrial complex I using BAY 87-2243 reduces melanoma tumor growth

Fig. 5

Vemurafenib increases mitochondrial density and respiration in BRAF mutant melanoma cells. a BRAF mutant melanoma cells were treated with various concentrations of vemurafenib. Cell proliferation was measured after 72 h. IC50 values were calculated using GraphPad prism (n = 4). b MitoTracker Green fluorescence (left) and quantification (right) (average intensity per cell) of BRAF mutant and BRAF wild type melanoma cells treated with vemurafenib (1 μM) for 72 h (n = 3). c OCR was measured using Seahorse analyzer in BRAF mutant and BRAF wild type melanoma cells after treatment with vemurafenib (1 μM) for 72 h (n = 3). d ATP-coupled OCR (after injection of oligomycin (1 μM)) was analyzed using Seahorse in BRAF mutant melanoma cells after treatment with vemurafenib (1 μM) for 72 h (n = 3). Data are represented as the mean ± SD. *p < 0.05

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