IDH1 loss suppresses contribution of glutamine carbon to lipogenesis under PDH suppression. a Western blot for PDH E1α and IDH1. Cyclophilin B was used as a loading control. b Percentage labeling in mass isotopologues of palmitate after 24 h of culture with [U-13C]glutamine. Inset displays labeling in lipogenic acetyl-CoA derived from glutamine carbon. c Cell count after 4 days of culture in normal serum. Data are the average of biological triplicates with error bars representing SD.