Fig. 5
From: Metabolic plasticity maintains proliferation in pyruvate dehydrogenase deficient cells
Metabolic flux analysis predicts formation of cytosolic citrate by reductive carboxylation of α-ketoglutarate. a Calculated PDH flux. b Calculated oxidative and reductive IDH fluxes. c Model of altered citrate metabolism in PDHA1-silenced cells. These changes are predicted by MFA using the INCA platform, as described in the text. Pathways in red are altered in PDHA1-silenced cells. Solid and dashed activities are predicted to be activated and suppressed, respectively. Data in panels a and b were derived from biological triplicates, with error bars representing 95 % CI. Abbreviations: AcCoA.c acetyl-CoA (cytosolic), Ac.CoA.m acetyl-CoA (mitochondrial), αKG α-ketoglutarate, Cit citrate, Gln glutamine, Glu glutamate, Mal malate, Ox oxidative, Pyr.c pyruvate (cytosolic), Pyr.m pyruvate (mitochondrial), Red reductive