Fig. 2
From: Metabolic plasticity maintains proliferation in pyruvate dehydrogenase deficient cells
Suppression of PDH activity reduces transfer of glucose carbon into citrate and lipids. a Schematic representing incorporation of 13C derived from glucose into metabolites of glycolysis and the TCA cycle. White and black circles are 12C and 13C, respectively. The solid line represents the first turn of the TCA cycle, and the dotted line represents the second turn. b Fractional abundance of citrate m + 2 and m + 4 during culture of H460 cells with [U-13C]glucose. c Fractional abundance of palmitate isotopologues after 24 h of culture in [U-13C]glucose. The inset shows the percentage of lipogenic acetyl-CoA derived from glucose carbon. d Labeling of lipid species from [U-14C]glucose. Data are the average and SD of biological triplicates, except for the inset in (c), where error bars represent 95 % CI. *P < 0.05; **P < 0.005. Abbreviations: CE cholesteryl esters, DAG diacylglycerol, FFA free fatty acids, Lac lactate, OAA oxaloacetate, PL phospholipids, Pyr pyruvate, Succ succinate, TAG triacylglycerol