Figure 4
From: Quantification of folate metabolism using transient metabolic flux analysis
Glutathione balance. (a) GSH synthesis and turnover. (b) GSH concentration (C GSH) 8 h after plating in untreated (open bars) and MTX-treated (filled bars) cells. (c) Fraction of unlabeled GSH. (d) Estimated GSH synthesis rate. (e) Estimated GSH turnover rate. (f) Estimated GSH consumption rate (g GSH = k GSH C GSH). Bars represent the average and error bars the standard deviation over three replicates. The symbol *X denotes a difference between untreated and MTX-treated with a statistical significance less than 5 × 10−X.