Figure 3
From: Quantification of folate metabolism using transient metabolic flux analysis
Energy stress and inhibition of protein synthesis. (a) AMP to ATP ratio in untreated cells (open white bars) and cells treated with MTX (filled blue bars). (b) ZMP levels. Not detected in untreated MCF7 cells. (c) Protein and phosphoprotein levels at different times after MTX treatment. (d,e) Ratio of phosphorylated to total protein levels using different scales for the Y-axis. (f) Protein synthesis rate estimated from the uptake of essential amino acids. Bars represent the average and error bars the standard deviation over three replicates. The symbol *X denotes a difference between untreated and MTX-treated with a statistical significance less than 5 × 10−X.