Figure 6

N-terminally tagged PHGDH forms larger macromolecular complexes than untagged PHGDH. (A) Representative images obtained from PHGDH and His-PHGDH specimens. Scale bar = 100 nm. (B) Montage of class averages of PHGDH and His-PHGDH sorted from smallest (top row) to largest (bottom row). Each average is 294 Å wide at the specimen level. (C) Normalized particle-size distributions (particles per bin/total particles) are displayed as histograms (dashed lines) and as smoothed curves (solid lines) for PHGDH (blue) and His-PHGDH (red). The His-PHGDH and PHGDH histograms are offset by 42 Ų to avoid overlapping lines.