Figure 5From: An epitope tag alters phosphoglycerate dehydrogenase structure and impairs ability to support cell proliferation N-terminal epitope-tagged and untagged PHGDH exhibit different mobility on size exclusion chromatography. Relative amount of rPHGDH and rHis-PHGDH protein present in fractions eluted from a Sephacryl S-200 size exclusion column. The 158- and 44-kDa standards are shown for comparison, with the column void volume represented in fraction 2.Back to article page