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Figure 2 | Cancer & Metabolism

Figure 2

From: An epitope tag alters phosphoglycerate dehydrogenase structure and impairs ability to support cell proliferation

Figure 2

PHGDH enzymatic activity is required for cell proliferation following PHGDH knockdown. (A) In vitro enzyme activity assessed by tracking NADH production by fluorescence. The assay was performed at saturating substrate concentrations. (B) Cell number over time for PHGDH-amplified T.T. cells stably expressing shRNA-resistant PHGDH wild type or R236E (enzymatically dead) cDNAs or empty vector (EV) control when infected with virus expressing GFP or PHGDH shRNA. Error bars show standard deviation from the mean.

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Cancer & Metabolism

ISSN: 2049-3002