Figure 5

In vivo effect of pyruvate pretreatment on improving TH-302 efficacy. Percent local tumor control of Hs766t (a), MiaPaCa-2 (b), and SU.86.86 (c) tumors were treated with saline, TH-302 alone (80 mg/kg × 5 days I.P.) or TH-302 following a 30 min pretreatment with exogenous pyruvate (1.15 mMol/kg pyruvate IV 30 min prior to 80 mg/kg TH-302 I.P. × 5 days). Response was measured as percentage of surviving animals as mice are removed from study when tumors reach 2,000 mm³. Pyruvate pretreatment significantly improved local control of Hs766t (p < 0.00225) and MiaPaCa-2 tumors with no measurable effect against SU.86.86. (d) Mean survival (days) of mice with pancreatic tumors treated with TH-302 and TH-302 in combination with pyruvate pre-treatment. N = 10 mice per treatment group. A two-tailed Student’s t-test was used to determine significance. **p < 0.01.