Figure 3
From: Pyruvate sensitizes pancreatic tumors to hypoxia-activated prodrug TH-302
Dynamic 13 C MR spectra following intravenous administration of pyruvate. 13C MR spectra from Hs766t (a), MiaPaCa-2 (c) and SU.86.86 (e) tumors. N=4 tumors per cell line. Pyruvate and lactate peaks are at 171 and 183 ppm respectively. Spectra were collected every 2 second. T2 weighted axial MR images containing slice ROI (red box) are inset. Tumor lactate and pyruvate peak intensities are shown with time for Hs766t (b), MiaPaCa-2 (d) and SU.86.86 (f) tumors. (g) Lactate to pyruvate ratio (Lac/Pyr) for all three tumor types. (N=4 tumors for each group and presented as mean ± S.D.). (h) Lactate Dehydrogenase A (LDHA) histological staining and (i) PPC analysis in all three PDAC tumors. Expression of LDHA was significantly greater in Hs766t and MiaPaCa-2 tumors in comparison to SU.86.86. Data are presented as mean ± S.D of four biological replicates. A two-tailed Student’s t-test was used to determine significance. * p<0.05. Scale Bar = 100 μM.