Figure 2From: Leucine supplementation differentially enhances pancreatic cancer growth in lean and overweight miceEffects of leucine supplementation on Panc02 tumor growth and apoptosis. (A) Differences in tumor volume between mice on control and calorie restriction (CR) diets with and without leucine (LEU) supplementation 4 weeks after tumor cell injections (control, control + LEU, and CR, n = 14/group; and CR + LEU, n = 13) (P <0.01 between control and both leucine-supplemented groups; P <0.001 between all other groups with different letters). (B) Comparison of immunohistochemical analyses performed on tumor sections for Ki-67 (n = 5/group; P <0.001 between all groups with different letters) and cleaved-caspase 3 (CC3) (n = 5/group; P <0.01 between the two leucine-supplemented groups; P <0.05 between all other groups with different letters). Scale bars represent 200 μm. Tumor volume is presented as mean ± SD, and Ki-67 and CC3 data are presented as mean ± SEM. Differences are considered significant if P <0.05. Abbreviations: CON, control diet; CR,calorie restriction diet; LEU, leucine-supplemented diet.Back to article page