Figure 1

Percentage ¹³C-labeling of cytosolic acetyl-CoA can be quantified from palmitate labeling. (A) Increasing ¹³C₂-acetyl-CoA labeling shifts palmitate labeling pattern to the right. ¹³C₂-acetyl-CoA labeling can be quantified by determining a best fit between observed palmitate labeling and computed binomial distributions (shown on right-hand side) from varying fractions of acetyl-CoA (AcCoA) labeling. (B) Steady-state palmitate labeling from U-¹³C-glucose and U-¹³C-glutamine in MDA-MB-468 cells. (C) Percentage acetyl-CoA production from glucose and glutamine. For (B) and (C), data are means ± SD of n = 3.