Percentage 13C-labeling of cytosolic acetyl-CoA can be quantified from palmitate labeling. (A) Increasing 13C2-acetyl-CoA labeling shifts palmitate labeling pattern to the right. 13C2-acetyl-CoA labeling can be quantified by determining a best fit between observed palmitate labeling and computed binomial distributions (shown on right-hand side) from varying fractions of acetyl-CoA (AcCoA) labeling. (B) Steady-state palmitate labeling from U-13C-glucose and U-13C-glutamine in MDA-MB-468 cells. (C) Percentage acetyl-CoA production from glucose and glutamine. For (B) and (C), data are means ± SD of n = 3.