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Figure 5 | Cancer & Metabolism

Figure 5

From: Succinate dehydrogenase inhibition leads to epithelial-mesenchymal transition and reprogrammed carbon metabolism

Figure 5

Carbon source utilization is altered upon Sdhb knockdown. (A) Mass spectrometry analysis of intracellular succinate, fumarate, and malate levels in Sdhb knockdown and scr control cells. Labeling of intracellular metabolites with (B) [U-13C]glucose or (C) [U-13C]glutamine was measured using mass spectrometry in Sdhb knockdown and scr control cells. Percent labeling of indicated isotopomers is shown. (D) Mass spectrometry analysis of extracellular glutamine in spent media of C1-scr and C1-sh1-Sdhb cells. Media was collected at 0 and 72 h. The y-axis depicts fold change of glutamine consumption relative to C1-scr cells. (E) Cells grown in normal media (1 mM glutamine) were switched to media with indicated concentrations of glutamine after 24 h. Cells were counted by trypan blue exclusion after 48 h. *p-value < .01.

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