Figure 3
From: Inhibition of 6-phosphofructo-2-kinase (PFKFB3) induces autophagy as a survival mechanism
Small molecule inhibition of PFKFB3 decreases glucose uptake and increases ROS in HCT-116 cells. HCT-116 cells were treated with 10 μM 3PO and cells were harvested at the indicated time points for the measurement of F2,6BP using an enzyme- coupled assay (A), 2DG uptake (B), ROS by DCFDA staining (C), ATP (D), and phospho-p70S6K, p70S6K, phospho-S6, S6, and β-actin by Western blot (E) and densitometry (F). Data are presented as the mean ± SD from three experiments (*P <0.05).