Figure 3

Loss of HSulf-1-mediated increase in fatty acids, sphingolipids, and lysolipids. The samples were extracted using Metabolon's standard solvent extraction method from cells in logarithmic phase having five biological replicates for each sample and distributed into equal parts for analysis on the GC/MS and LC/MS/MS platforms. (A) Fold increase of long-chain fatty acids were calculated by the average metabolite level of Sh1/NTC and Sh2/NTC. *p = 0.02 to 0.09; **p = 0.002 to 0.01; ***p < 0.001 compared to NTC. (B) Branched chain fatty acids and (C) essential fatty acids were calculated by the average metabolite level of Sh1/NTC and Sh2/NTC. p values for both branched chain fatty acids and essential fatty acids were *p = 0.01 to 0.02; **p = 0.002 to 0.01; ***p < 0.001 compared to NTC. (D) Fold increase of sphingolipids were calculated by the average metabolite level of Sh1/NTC and Sh2/NTC. *p = 0.01 to 0.09; **p = 0.002 to 0.008; ***p < 0.001 compared to NTC. (E) Choline/inositol pathway metabolites' fold change and (F) membrane lysolipids were calculated as mentioned earlier. P values for choline/inositol pathway was *p = 0.009 to 0.09; **p = 0.002 to 0.008; ***p < 0.001 whereas *p = 0.01 to 0.02; **p = 0.002 to 0.009; ***p < 0.001 for membrane lysolipids compared to NTC.