Figure 3From: GLUT3 is induced during epithelial-mesenchymal transition and promotes tumor cell proliferation in non-small cell lung cancerGLUT3 is strongly expressed in mesenchymal liver tumor cells. (A) The indicated cell lines were lysed for RNA preparation followed by reverse transcription. The cDNA was amplified by real-time PCR using probes specific for the indicated genes or internal controls. Data show means ± s.d. (n = 3) of mRNA expression relative to the cell line expressing the least amount of the same gene (set to 1). N.D., not detected. (B) Hep3B cells were stimulated with 10 ng/ml TGF-β for 72 h, after which the cells were lysed for RNA preparation followed by reverse transcription. The cDNA was amplified by real-time PCR using probes specific for the indicated genes or internal controls. Data show means ± s.d. (n = 3) of mRNA expression relative to the untreated conditions (set to 1).Back to article page