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Figure 2 | Cancer & Metabolism

Figure 2

From: GLUT3 is induced during epithelial-mesenchymal transition and promotes tumor cell proliferation in non-small cell lung cancer

Figure 2

GLUT3 is induced upon EMT. (A) H2122 cells were stimulated with 10 ng/ml TGF-β for the indicated time points, after which the cells were lysed for RNA preparation followed by reverse transcription. The cDNA was amplified by real-time PCR using probes specific for the indicated genes or internal controls. Data show means ± s.d. (n = 4) of mRNA expression ratios between TGF-β treated and non-treated conditions. (B) H727 or H2009 cell populations stably expressing a control plasmid (ctl), SNAIL, or ZEB1 were lysed for RNA preparation followed by reverse transcription. The cDNA was amplified by real-time PCR using probes specific for the indicated genes or internal controls. Data show means ± s.d. (n = 3) of mRNA expression relative to the cell line expressing the least amount of the same gene (set to 1). (C) H727 or H2009 stable cell populations were lysed with RIPA buffer to prepare protein extracts and to analyze the expression of the indicated proteins by Western blot.

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