Bioenergetic profiles of osteocytes and MYC-dependent osteogenic sarcoma cells. (a) Oxygen consumption rate (OCR) was determined using a Seahorse Bioscience XF24 Flux Analyzer by sequential injection of oligomycin, FCCP and antimycin A/rotenone. (b) Basal, coupled, maximal, and uncoupled oxygen consumption rate (OCR) was assessed in osteogenic sarcoma cells in the presence or absence of 20 ng/mL doxycycline for 48 hours. N = 6 ± SEM * P < 0.05 compared to 0 ng/ml doxycycline. (c) Spare respiratory capacity defined as maximal respiration minus basal respiration. N = 6 ± SEM * P < 0.05 compared to 0 ng/ml doxycycline. (d) Mitochondrial membrane potential as assessed by TMRE mean fluorescence intensity (MFI), corrected by FCCP. N = 5 ± SEM * P<0.05 compared to 0 ng/ml doxycycline.