Osteogenic sarcoma cells differentiate into mature osteocytes under MYC suppression. (a) MYC protein levels in osteogenic sarcoma cells treated with doxycycline (20 ng/mL) at 24, 48, 72 and 96 hours. (b) Phase contrast microscopy pictures of osteogenic sarcoma cells after 24 and 48 hours treatment with doxycycline (20 ng/mL). (c) Cell cycle analysis was performed by staining the cells with propidium iodide (PI) after 48 hours doxycycline (20ng/ml) treatment. N = 4 ± SEM * P < 0.05 compared to 0 ng/ml doxycycline.