Figure 3
ERRα modulates glutamine metabolism in normoxia and hypoxia. (A) Expression of glutamine metabolism genes in SK-BR-3 cells treated with ERRα inhibitor C29 and cultured in either normoxia or hypoxia for 24 h. Expression is normalized to normoxic control cells. Data are represented as means ± S.E.M., from three biological replicates representative of two independent experiments. *P <0.05, unpaired Student's t-test. (B) Expression of glutamine metabolism genes in BT-474 cells treated with C29 and cultured in either normoxia or hypoxia for 24 h. Expression is normalized to normoxic control cells. Data are represented as means ± S.E.M., from four independent cultures. *P <0.05, paired Student's t-test. (C) Mass isotopomer distribution analysis of CAC intermediates in SK-BR-3 and BT-474 cells treated as in A and B, respectively, and pulsed with [U-13C]-glutamine. For SK-BR-3, data are represented as means ± S.E.M., from three biological replicates representative of two independent experiments. *P <0.05, unpaired Student's t-test. For BT-474, data are represented as means ± S.E.M., from four independent cultures. *P <0.05, paired Student's t-test. For panel C, relative ion levels represent specific mass isotopomer fractions normalized to that of control cells in normoxia.