Figure 6

Effects of fibroblast growth factor receptor (FGFR)4 deficiency on systemic adipokine levels in tumor-bearing mice. (A) Antibody-based array analyses. The panel of adipokines as indicated was measured simultaneously in sera from the MMTV-TGFα transgenic (Tg) and FGFR4^−/−: MMTV-TGFα bigenic (KO-Tg) mice (n = 8 for each group) at 6 months. Changes of adipokine levels were expressed as log of the densitometric ratio of KO-Tg to Tg. Data are means ± SD; P <0.05. (B-D) Changes in expression of liver FGF21, ileal FGF15 and breast adiponectin (ADIPOQ) in the wild-type (WT), FGFR4 knockout (KO), Tg and KO-Tg mice at 6 months were determined by quantitative PCR. Results were normalized to the β-actin levels in the respective samples and then expressed as fold change relative to that of WT, which is assigned as an arbitrary unit of 1 or 100. Data are means ± SD, ^*P <0.05 (n = 6 for each group).